Premier Experimental Design - Revision history

Admin at 10:26, 30 August 2024

2024-08-30T10:26:29Z

Admin at 10:25, 30 August 2024

2024-08-30T10:25:16Z

Admin: /* Target Parameter */

2021-01-15T12:30:17Z

Target Parameter

New page

==Introduction==
This template should help you to plan your project in such a way that all possible difficulties, risks and systematic errors that may occur are considered and minimized in advance. By means of specific questions the ever present bias in experiments shall be reduced and project specific topics shall be brought into the awareness of your experimental design. It should be noted that you do not necessarily have to / can answer all points, which depends on your type of project (exploratory / confirmatory etc.). If you can't say anything about single points, please briefly explain why.

Explainer videos for technical use can be found [https://premier-qms.org/premier/planning-of-experiments/template-usage here].

== Project details ==

'''Project name:'''

'''Project Manager:'''

'''Planned project duration:'''

'''Short project description:''' (What is your project about? What is the goal?)

(If required, click the above edit button and upload an image.)

== Search ==
[https://premier-qms.org/premier/planning-of-experiments/search support]

'''Literature Databases / Other sources:'''

'''What is (was) the research strategy?'''

== Hypothesis / Counterhypothesis ==

[https://premier-qms.org/premier/planning-of-experiments/hypothesis-counter-hypothesis support]

'''Set up hypothesis / counter hypothesis: Have you formulated your hypothesis and the corresponding counter and/or null hypothesis?'''

'''Counterhypothesis: Have you searched for literature that argues against the hypothesis?'''

== Target Parameter ==

[https://premier-qms.org/premier/planning-of-experiments/target-parameters support]

Contact person for:

In vivo questions: André Rex

In vitro questions: Dorette Freyer

'''Definition of the target parameters: Which primary and secondary target parameters have you defined?'''

== Sample Size Calculation ==

[https://premier-qms.org/premier/planning-of-experiments/sample-size-calculation support]

'''Need for experimental units: How was the need for experimental units (number of animals, organs, organ sections or cultured cells) determined?'''

'''Which reference was used for the estimation? groups and units: Name the experimental groups with the exact number of units and identify test and control groups.'''

== Experimental Design / Model Planning ==

[https://premier-qms.org/premier/planning-of-experiments/model-planning-study-design support]

'''Definition of criteria: Have you defined the following criteria for your project: - method selection - influencing factors - requirements - control groups - validations?'''

== Feasibility Check ==

[https://premier-qms.org/premier/planning-of-experiments/feasibility-study support]

'''Feasibility of the project: At the end of the feasibility study there is a plausibility check. Is it positive for your project?'''

== Nesting and Pseudoreplication ==

[https://premier-qms.org/premier/planning-of-experiments/nesting-and-pseudoreplication support]

René Bernard will help you with this topic.

'''Identification of the problem: Does the biological unit used for statistical evaluation correspond to the one used for randomisation? If not, check if pseudoreplication is present and how it can be avoided in the evaluation.'''

== Randomization and Blinding ==

[https://premier-qms.org/premier/planning-of-experiments/randomisation-and-blinding support]

René Bernard will help you with this topic.

'''Implementation of R+B: Which methods were used to randomize and blind your experiments?'''

'''If you did not perform randomization and blinding, please explain why!'''

== Resource Plan ==

[https://premier-qms.org/premier/planning-of-experiments/resource-plan-financing-capacities-personnel support]

'''Resources and infrastructure: Is the financing of your project secured and is there enough personnel, material and a suitable infrastructure available?'''

'''Alternatives: If this is not the case, are there planned strategies to overcome the possible challenges?'''

==Timetable==
[https://premier-qms.org/premier/planning-of-experiments/schedule support]

'''Preparation of the timetable: Have you created a detailed, realistic timetable for your project?'''

'''Is a project management tool available?'''

'''How do you manage, review and update this timetable?'''

(If required, click the above edit button and upload a timetable Excel file.)

== Accompanying training courses ==

[https://premier-qms.org/premier/planning-of-experiments/accompanying-training-and-courses support]

'''Determine training needs: Are there any special trainings, courses etc. that are absolutely necessary in order to carry out the project?'''

'''Attend training courses: Do you attend training courses that can be helpful for your project?'''

== Planning of Data Preparation / Analysis ==

[https://premier-qms.org/premier/planning-of-experiments/planning-of-data-preparation-analysis support]

'''Data analysis: How did you plan the aggregation and preparation of the data?'''

'''Please explain and document (in the ELN) the step from primary to secondary data!'''

'''Archiving the data: Have you ensured the archiving of primary and secondary data?'''

== Data Storage ==

[https://premier-qms.org/premier/planning-of-experiments/data-storage support]

'''Location: Where did you store the primary and secondary data?'''

'''Backups: Are you planning additional backups?'''

== Clarification of Authorships ==

[https://premier-qms.org/premier/planning-of-experiments/clarification-of-authorship support]

'''Requirements for first authorship: How are the authorships for your project clarified?'''

'''Documentation: Has the agreement been documented? If so, where?'''

== Preregistration ==

[https://premier-qms.org/premier/planning-of-experiments/pre-registration support]

René Bernard and Claudia Kurreck also provide further assistance on this topic.

'''Implementation: Where did you pre register your project?'''

'''Please explain in the ELN if you have not pre registered your project!'''

@@ Line 1: / Line 1: @@
-==Introduction==
+<h2>Introduction</h2>This template should help you to plan your project in such a way that all possible difficulties, risks and systematic errors that may occur are considered and minimized in advance. By means of specific questions the ever present bias in experiments shall be reduced and project specific topics shall be brought into the awareness of your experimental design. It should be noted that you do not necessarily have to / can answer all points, which depends on your type of project (exploratory / confirmatory etc.). If you can't say anything about single points, please briefly explain why.
 Explainer videos for technical use can be found [https://premier-qms.org/premier/planning-of-experiments/template-usage here].
-==Project details==
 '''Project name:'''
@@ Line 18: / Line 18: @@
 (If required, click the above edit button and upload an image.)
-==Search==
 [https://premier-qms.org/premier/planning-of-experiments/search support]
 '''Literature Databases / Other sources:'''
@@ Line 27: / Line 28: @@
-==Hypothesis / Counterhypothesis==
+<h2>Hypothesis / Counterhypothesis</h2>[https://premier-qms.org/premier/planning-of-experiments/hypothesis-counter-hypothesis support]
 '''Set up hypothesis / counter hypothesis: Have you formulated your hypothesis and the corresponding counter and/or null hypothesis?'''
@@ Line 36: / Line 36: @@
-==Target Parameter==
+<h2>Target Parameter</h2>[https://premier-qms.org/premier/planning-of-experiments/target-parameters support]
 ''''''
-==Sample Size Calculation==
+<h2>Sample Size Calculation</h2>[https://premier-qms.org/premier/planning-of-experiments/sample-size-calculation support]
 '''Need for experimental units: How was the need for experimental units (number of animals, organs, organ sections or cultured cells) determined?'''
@@ Line 53: / Line 51: @@
-==Experimental Design / Model Planning==
+<h2>Experimental Design / Model Planning</h2>[https://premier-qms.org/premier/planning-of-experiments/model-planning-study-design support]
 '''Definition of criteria: Have you defined the following criteria for your project: - method selection - influencing factors - requirements - control groups - validations?'''
-==Feasibility Check==
+<h2>Feasibility Check</h2>[https://premier-qms.org/premier/planning-of-experiments/feasibility-study support]
 '''Feasibility of the project: At the end of the feasibility study there is a plausibility check. Is it positive for your project?'''
-==Nesting and Pseudoreplication==
+<h2>Nesting and Pseudoreplication</h2>[https://premier-qms.org/premier/planning-of-experiments/nesting-and-pseudoreplication support]
@@ Line 73: / Line 68: @@
-==Randomization and Blinding==
+<h2>Randomization and Blinding</h2>[https://premier-qms.org/premier/planning-of-experiments/randomisation-and-blinding support]
@@ Line 84: / Line 78: @@
-==Resource Plan==
+<h2>Resource Plan</h2>[https://premier-qms.org/premier/planning-of-experiments/resource-plan-financing-capacities-personnel support]
 '''Resources and infrastructure: Is the financing of your project secured and is there enough personnel, material and a suitable infrastructure available?'''
@@ Line 93: / Line 86: @@
-==Timetable==
+<h2>Timetable</h2>[https://premier-qms.org/premier/planning-of-experiments/schedule support]
 '''Preparation of the timetable: Have you created a detailed, realistic timetable for your project?'''
@@ Line 106: / Line 98: @@
 (If required, click the above edit button and upload a timetable Excel file.)
-==Accompanying training courses==
 [https://premier-qms.org/premier/planning-of-experiments/accompanying-training-and-courses support]
 '''Determine training needs: Are there any special trainings, courses etc. that are absolutely necessary in order to carry out the project?'''
@@ Line 115: / Line 108: @@
-==Planning of Data Preparation / Analysis==
+<h2>Planning of Data Preparation / Analysis</h2>[https://premier-qms.org/premier/planning-of-experiments/planning-of-data-preparation-analysis support]
 '''Data analysis: How did you plan the aggregation and preparation of the data?'''
@@ Line 127: / Line 119: @@
-==Data Storage==
+<h2>Data Storage</h2>[https://premier-qms.org/premier/planning-of-experiments/data-storage support]
 '''Location: Where did you store the primary and secondary data?'''
@@ Line 136: / Line 127: @@
-==Clarification of Authorships==
+<h2>Clarification of Authorships</h2>[https://premier-qms.org/premier/planning-of-experiments/clarification-of-authorship support]
 '''Requirements for first authorship: How are the authorships for your project clarified?'''
@@ Line 145: / Line 135: @@
-==Preregistration==
+<h2>Preregistration</h2>[https://premier-qms.org/premier/planning-of-experiments/pre-registration support]

@@ Line 4: / Line 4: @@
 Explainer videos for technical use can be found [https://premier-qms.org/premier/planning-of-experiments/template-usage here].
-== Project details ==
+==Project details==
 '''Project name:'''
@@ Line 19: / Line 18: @@
 (If required, click the above edit button and upload an image.)
-== Search ==
+==Search==
 [https://premier-qms.org/premier/planning-of-experiments/search support]
@@ Line 28: / Line 27: @@
-== Hypothesis / Counterhypothesis ==
+==Hypothesis / Counterhypothesis==
 [https://premier-qms.org/premier/planning-of-experiments/hypothesis-counter-hypothesis support]
@@ Line 38: / Line 36: @@
-== Target Parameter ==
+==Target Parameter==
 [https://premier-qms.org/premier/planning-of-experiments/target-parameters support]
-Contact person for:
+''''''
 '''Definition of the target parameters: Which primary and secondary target parameters have you defined?'''
-== Sample Size Calculation ==
+==Sample Size Calculation==
 [https://premier-qms.org/premier/planning-of-experiments/sample-size-calculation support]
@@ Line 60: / Line 53: @@
-== Experimental Design / Model Planning ==
+==Experimental Design / Model Planning==
 [https://premier-qms.org/premier/planning-of-experiments/model-planning-study-design support]
@@ Line 67: / Line 59: @@
-== Feasibility Check ==
+==Feasibility Check==
 [https://premier-qms.org/premier/planning-of-experiments/feasibility-study support]
@@ Line 74: / Line 65: @@
-== Nesting and Pseudoreplication ==
+==Nesting and Pseudoreplication==
 '''Identification of the problem: Does the biological unit used for statistical evaluation correspond to the one used for randomisation? If not, check if pseudoreplication is present and how it can be avoided in the evaluation.'''
-== Randomization and Blinding ==
+==Randomization and Blinding==
 '''Implementation of R+B: Which methods were used to randomize and blind your experiments?'''
@@ Line 95: / Line 84: @@
-== Resource Plan ==
+==Resource Plan==
 [https://premier-qms.org/premier/planning-of-experiments/resource-plan-financing-capacities-personnel support]
@@ Line 118: / Line 106: @@
 (If required, click the above edit button and upload a timetable Excel file.)
-== Accompanying training courses ==
+==Accompanying training courses==
 [https://premier-qms.org/premier/planning-of-experiments/accompanying-training-and-courses support]
@@ Line 128: / Line 115: @@
-== Planning of Data Preparation / Analysis ==
+==Planning of Data Preparation / Analysis==
 [https://premier-qms.org/premier/planning-of-experiments/planning-of-data-preparation-analysis support]
@@ Line 141: / Line 127: @@
-== Data Storage ==
+==Data Storage==
 [https://premier-qms.org/premier/planning-of-experiments/data-storage support]
@@ Line 151: / Line 136: @@
-== Clarification of Authorships ==
+==Clarification of Authorships==
 [https://premier-qms.org/premier/planning-of-experiments/clarification-of-authorship support]
@@ Line 161: / Line 145: @@
-== Preregistration ==
+==Preregistration==
 '''Implementation: Where did you pre register your project?'''